Pengaruh Pemberian Ekstrak Etanol Daun Sisik Naga (Pyrrosia Piloselloides L.) terhadap Penurunan Kadar Kolesterol Total Mencit Hiperkolesterolemia
Effect of Dragon Scales Leaf Ethanol Extract (Pyrrosia Piloselloides L.) on Reducing Total Cholesterol Levels in Hypercholesterolemic Mice
DOI:
https://doi.org/10.25026/jsk.v5i4.1582Keywords:
: Hiperkolesterolemia, Pyrrosia piloselloides L., MDLT, Kadar kolesterol totalAbstract
Hypercholesterolemia is one of the risk factor for cardiovascular disease such as stroke, atherosclerosis and coronary heart disease. The purpose of this study was to determine the effect of ethanol extract of dragon scales leaves (Pyrrosia piloselloides L.) on reducing total cholesterol (TC) of test animals. The instrument to test was 25 male white mice were induced hypercholesterolemia by eating a high fat diet (MDLT) in around 14 days. The animals were declared as hypercholesterolemia if the total of cholesterol level (TC) more than 230 mg/dl. Then the Animals divided into five groups namely positive control, comparison (simvastatin), and the group given the test extract doses of 25, 50, 100 mg/kgBB. The treatment was given for 15 days, and TC level were checked on days 0, 5, 10 and 15. The data were analyzed by Two Way of ANOVA and continued with Duncan's test. The results showed that the dose of the tested extract and the duration of administration had a significant effect (p<0.05) on reduce in TC levels of the test animals. All variations in the doses of ethanol extract from dragon scale leaves had the effect to reduce TC in the animals and the percentage reduction value was equal to the group given simvastatin. These results indicate that the ethanol extract of dragon scales leaves has the potential to reduce total cholesterol levels in hypercholesterolemic mice.Keywords: Hypercholesterolemia, Pyrrosia piloselloides L., High-fat diet, Total cholesterol levels
Abstrak
Hiperkolesterolemia merupakan faktor risiko terjadinya penyakit kardiovaskular seperti strok, aterosklerosis dan jantung koroner. Tujuan dari penelitian ini untuk mengetahui pengaruh ekstrak etanol daun sisik naga (Pyrrosia piloselloides L.). Sebanyak 25 ekor mencit putih jantan diinduksi hiperkolesterolemia dengan makanan diet lemak tinggi (MDLT) ±14 hari, mencit dinyatakan hiperkolesterolemia jika kadar kolesterol total (KT) >230mg/dl, kemudian dibagi menjadi 5 kelompok perlakuan yaitu kontrol positif, pembanding (simvastatin), dan kelompok yang diberi ekstrak uji dosis 25, 50, 100 mg/kgBB. Perlakuan diberikan selama 15 hari, pada hari ke 0, 5, 10 dan 15 dilakukan pemeriksaan kadar KT. Data dianalisis dengan Two Way ANOVA dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan dosis ekstrak uji dan waktu berpengaruh signifikan (p<0,05) terhadap penurunan kadar KT mencit. Semua kelompok dosis ekstrak etanol daun sisik naga memberikan efek penurunan KT pada mencit dan nilai persentase penurunannya sebanding dengan kelompok yang diberi simvastatin. Hasil ini menunjukkan ekstrak etanol daun sisik naga berpotensi dalam menurunkan kadar kolesterol total mencit hiperkolesterolemia
Kata Kunci: Hiperkolesterolemia, Pyrrosia piloselloides L., MDLT, Kadar kolesterol total
References
Luo, J., Jiang, L. Y., Yang, H. Y. dan Song, B. L. 2019. Intracellular cholesterol transport by sterol transfer proteins at membrane contact sites. Trends Biochem. Sci.44, 273–292.
Priya.T, Shashank,M.,and Kishwar Hayat Khan. 2013. Cholesterol: Genetic, Clinical and Natural Implications. Journal of Pharmaceutical, Biological and Chemical Sciences. ISSN: 0975-8585.
Abdel, H. A., Rakeel, H. A., Rada, H. A. 2018. Hypercholesterolemia as a risk factor for coronary heart disease. J. Of University Of Anbar For Pure Science : Vol.12 : No.3. ISSN: 1991-8941.
Beltowski, J. 2014. Reverse epidemiology in ischemic stroke: highcholesterol as a predictor of improved survival in stroke patients, Clinical Lipidology, 9:2, 135-139.
World Helth Organization (WHO). 2017. Cardiovascular diseases (CVDs). Diunduh dari: https://www.who.int/news-room/fact-sheets/detail/cardiovascular-diseases-(cvds).
Atshaves ,B. P., G. G. Martin, H. A. Hostetler, A. L. McIntosh, A. B. Kier, and F. Schroeder. 2010. Liver fatty acid-binding protein and obesity. Journal of Nutritional Biochemistry. vol. 21, no. 11, pp. 1015–1032.
Libby P. 2015. The Pathogenesis, Prevention, and Treatment of Atherosclerosis. New York: McGraw-Hill Education; 291e1–291e10.
Onuah,C.L., Anacletus, F.C., Uwakwe, A.A., Okoroh,P.N. 2018. The Synergistic Effect Of Ethanol Leaf Extract Of Annona Muricata And Artocarpus Heterophyllus On High Fat Diet Induced Hyperlipidaemia In Wistar Albino Rats. World journal of pharmaceutical research. Vol 7, no 19, 52-60.
Malinda, A. F dkk. 2013. Pengaruh Pemberian Ekstrak Etanol Daun Paku Sisik Naga (Drymoglossum Piloselloides L.Presl) Terhadap Peroksidasi Lipid Hati Pada Tikus Jantan Galur Wistar Yang Diinduksi CCl4. Jurnal Ilmiah Farmasi. Vol. 2 No. 02.
Arifin H., dkk. 2017. Pengaruh Pemberian Ekstrak Etanol Daun Sisik Naga (Pyrrosia Piloselloides (L.) M. G Price) terhadap Waktu Pendarahan, Waktu Pembekuan Darah dan Jumlah Trombosit Mencit Putih Jantan. Jurnal Farmasi Higea, Vol. 9, No. 1.
Somchit M. N., Hassan H., Zuraini A., Chong L. C., Mohamed Z., Zakaria Z. A. 2011. In vitro anti-fungal and anti-bacterial activity of Drymoglossum piloselloides L. Presl. against several fungi responsible for Athlete's foot and common pathogenic bacteria. Afr J Microbiol Res .5(21):3537-3541.
Arief, M. Z .2018. Phytochemical Screening and Toxicological Evaluation of Pyrrosia piloselloides Extracts. International Medical Journal .Vol. 25, No. 3, pp. 177 – 180.
Lee K. H., Park E., Lee H. J., et al. 2011. Effects of daily quercetin-rich supplementation on cardiometabolic risks in male smokers. Nutr Res Pract; 5(1):28-33.
Zeka K, et al. 2017. Flavonoids and Their Metabolites: Prevention in Cardiovascular Diseases and Diabetes. Journal Diseases, 5, 19; doi:10.3390.
Harborne, J.B. 1987. Metode Fitokimia. Penuntun Cara Modern Menganalisis Tumbuhan. terbitan kedua. ITB. Bandung, Indonesia.
Armenia, Ermilda, F., Widya R.M., Rusdji D.J., Netty M. 2006. Anti-Atheroschelrotic Effect and Liver Toxicity of Ethanolic Extract of Phaleria Macrocarpa (Scheff) Boerl Fruit on Japanese Quail. Oral paper ASOMP XII, 13-18.
Zhou H, Hylemon PB. 2014. Bile acids are nutrient signaling hormones. Steroids; 86: 62-8.
Santana Farre,R. et al. 2012. Influence of neonatal hypothyroidism on hepatic gene expression and lipid metabolism in adulthood. PLOS ONE 7, e37386.
Montoya C, Cochard B, Flori A, Cros D, Lopes R, et al. (2014) Genetic Architecture of Palm Oil Fatty Acid Composition in Cultivated Oil Palm (Elaeis guineensis Jacq.) Compared to Its Wild Relative E. Oleifera (H.B.K) Corte ´s. PLoS ONE 9(5): e95412. doi: 10.1371/journal.pone.0095412.
Patty W. Siri Tarino, Q. S. 2010. Saturated Fatty Acids and Risk of Coronary Heart Disease: Modulation by Replacement Nutrients. Current Atheroscler Reports, 12:384–390.
Huy L A P, Hua, H., Chuong P H. 2008. Free Radicals, Antioxidants in Disease and Health. International journal of Biomedical science. vol. 4 no. 2.
Raphael J. Ogbe et al. 2015. A review on dietary phytosterols:Their occurrence, metabolism and health benefits. Asian Journal of Plant Science and Research, 2015, 5(4):10-21.
Amany M.M. Ali*, Tawfik M.F., Hikal M.S. And Tag El-Din M.A. 2019. Hypocholesterolemic Effect Of Saponin Extracts In Experimental Animals. Arab Univ. Journal. Agric. Sci. (Aujas). 2463-2478.
Aronson, Jeffrey K,. 2007. Concentration-effect and dose-response relations in clinical pharmacology. British Journal of Clinical Pharmacology. DOI:10.1111/j.1365-2125.2007.02871.
Joudi,A, F,S. 2013. Adverse Effects of Excessive Antioxidant Supplements and Their Underlying Mechanisms. Journal Of Aging Research & Clinical Practice. Vol 2(4).
Vakil, V, Wade Trappe. Drug Combinations: Mathematical Modeling and Networking Methods. Journal Pharmaceutical. 11, 208; doi:10.3390.
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